Rats are euthanized as per IACUC guidelines

Spray fur with ethanol. Pick up skin above the sternum, and make a cut through skin and muscle layers.

Don’t cut the chest cavity (don’t go above the diaphragm).

Cut skin and muscles from the sternum down to the lower belly. Mostly you will see liver and colon.

Lift liver lobes and push aside fat tissue and intestines. You will see dark-colored organs – kidney and spleen.

Count the number of observed kidneys and make a record in the file.