Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip

Hans Clevers, Linda Gijzen, Fjodor A. Yousef Yengej, Frans Schutgens, Marianne K. Vormann, Carola M. E. Ammerlaan, Arnaud Nicolas, Dorota Kurek, Paul Vulto, Maarten B. Rookmaaker, Henriette L. Lanz, Marianne C. Verhaar

Published: 2021-03-04 DOI: 10.1038/s41596-020-00479-w

organ-on-a-chip

microfluidic

kidney tubuloids

renal physiology

in vitro model

AI 解读

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Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Advanced invitro kidney models are of great importance to the study of renal physiology and disease. Kidney tubuloids can be established from primary cells derived from adult kidney tissue or urine. Tubuloids are three-dimensional multicellular structure
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS

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Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip

Extended

Extended Data Fig. 1 Expression levels of markers of the different nephron segments are stable in tubuloids over multiple passages, except for a decrease in the loop of Henle–specific transporter SLC12A1/NKCC2 .

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