Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Carlos A. Tristan, Hyenjong Hong, Yogita Jethmalani, Yu Chen, Claire Weber, Pei-Hsuan Chu, Seungmi Ryu, Vukasin M. Jovanovic, Inae Hur, Ty C. Voss, Anton Simeonov, Ilyas Singeç

Published: 2022-10-18 DOI: 10.1038/s41596-022-00753-z

microfluidics

hPSCs

single-cell cloning

gene editing

CEPT cocktail

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Human pluripotent stem cells (hPSCs) are inherently sensitive cells. Single-cell dissociation and the establishment of clonal cell lines have been long-standing challenges. This inefficiency of cell cloning represents a major obstacle for the standardizat
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
Limitations
- Reporting summary
- References
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

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