Engineering SARS-CoV-2 using a reverse genetic system

Xuping Xie, Kumari G. Lokugamage, Xianwen Zhang, Michelle N. Vu, Antonio E. Muruato, Vineet D. Menachery, Pei-Yong Shi

Published: 2021-01-28 DOI: 10.1038/s41596-021-00491-8

SARS-CoV-2

reverse genetics

virus engineering

cDNA clone

COVID-19 research

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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Reverse genetic systems are a critical tool for studying viruses and identifying countermeasures. In response to the ongoing COVID-19 pandemic, we recently developed an infectious complementary DNA (cDNA) clone for severe acute respiratory syndrome corona
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS

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Menachery, V. D. et al. Combination attenuation offers strategy for live attenuated coronavirus vaccines. J. Virol. http://doi.org/10.1128/JVI.00710-18 (2018).
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Engineering SARS-CoV-2 using a reverse genetic system

Supplementary information

Supplementary Information

Reporting Summary

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