球客岛官网(中国)

    Intraperitoneal L-dopa injection in mice

    natalia.lopezgonzalezdelrey, Zachary Gaertner

    Published: 2023-06-17 DOI: 10.17504/protocols.io.81wgbxd51lpk/v1
    AI 解读
    浏览 1358

    Abstract

    Intraperitoneal L-dopa injection in mice

    Steps

    Preparation

    1.

    Mice were acclimated to the room for 1h 0m 0s

    L-dopa preparation

    2.

    Prepare the L-dopa mix fresh every day.

    Prepare a master mix of: 12.5mg/kg L-dopa and 12.5mg/kg benserazide dissolved in sterile saline with 0.25% citrate (wt/vol). Calculate grams depending on number of mice to be injected. Using vortex will dissolve the solution easier.

    L-dopa injection

    3.

    Mice are injected intraperitoneally with the 12.5mg/kg L-dopa and 12.5mg/kg benserazide solution.

    Behaviour

    4.

    One hour post injection, mice were tested using the appropiate behavior protocol.

    推荐阅读

    Nature Protocols
    Protocols IO
    Current Protocols
    扫码咨询