Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors

Robert Zweigerdt, Mania Ackermann, Anna Rafiei Hashtchin, Felix Manstein, Marco Carvalho Oliveira, Henning Kempf, Nico Lachmann

Published: 2022-01-16 DOI: 10.1038/s41596-021-00654-7

induced pluripotent stem cells

macrophage mass production

suspension culture

stirred-tank bioreactors

cell-based therapies

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Macrophages derived from human induced pluripotent stem cells (iPSCs) have the potential to enable the development of cell-based therapies for numerous disease conditions. We here provide a detailed protocol for the mass production of iPSC-derived macroph
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
References
- Additional materials required
- Procedure
ACKNOWLEDGMENTS

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Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors

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