Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers

Vera M. Titze, Soraya Caixeiro, Vinh San Dinh, Matthias König, Matthias Rübsam, Nachiket Pathak, Anna-Lena Schumacher, Maximilian Germer, Christian Kukat, Carien M. Niessen, Marcel Schubert, Malte C. Gather

Published: 2024-01-18 DOI: 10.1038/s41596-023-00924-6

bio-integrated microlasers

hyperspectral confocal imaging

high-throughput readout

intracellular biosensing

multiplexed cell tracking

AI 解读

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

查看全部

Sections

Figures

References

Kavčič, A. et al. Deep tissue localization and sensing using optical microcavity probes. Nat. Commun. 13, 1269 (2022).
Champion, J. A. & Mitragotri, S. Role of target geometry in phagocytosis. Proc. Natl Acad. Sci. USA 103, 4930–4934 (2006).
Su, J. Label-free biological and chemical sensing using whispering gallery mode optical resonators: past, present, and future. Sens. (Basel) 17, 540 (2017).
Schubert, M. et al. Monitoring contractility in cardiac tissue with cellular resolution using biointegrated microlasers. Nat. Photonics 14, 452–458 (2020).
Schubert, M. et al. Lasing within live cells containing intracellular optical microresonators for barcode-type cell tagging and tracking. Nano Lett. 15, 5647–5652 (2015).
Humar, M. & Yun, S. H. Intracellular microlasers. Nat. Photonics 9, 572–576 (2015).
Schubert, M. et al. Lasing in live mitotic and non-phagocytic cells by efficient delivery of microresonators. Sci. Rep. 7, 40877 (2017).
Li, X. et al. In vivo tracking of individual stem cells labeled with nanowire lasers using multimodality imaging. Biomed. Opt. Express 13, 4706–4717 (2022).
Martino, N. et al. Wavelength-encoded laser particles for massively multiplexed cell tagging. Nat. Photonics 13, 720–727 (2019).
Foreman, M. R., Swaim, J. D. & Vollmer, F. Whispering gallery mode sensors. Adv. Opt. Photonics 7, 168–240 (2015).
Titze, V. M., Caixeiro, S., Di Falco, A., Schubert, M. & Gather, M. C. Red-shifted excitation and two-photon pumping of biointegrated GaInP/AlGaInP quantum well microlasers. ACS Photonics 9, 952–960 (2022).
Gather, M. C. & Yun, S. H. Single-cell biological lasers. Nat. Photonics 5, 406–410 (2011).
Wei, Y. et al. Starch-based biological microlasers. ACS Nano 11, 597–602 (2017).
Humar, M. & Yun, S. H. Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies. Optica 4, 222–228 (2017).
Lee, S. S., Kim, J. B., Kim, Y. H. & Kim, S.-H. Wavelength-tunable and shape-reconfigurable photonic capsule resonators containing cholesteric liquid crystals. Sci. Adv. 4, 8276–8298 (2018).
Hales, J. E., Matmon, G., Dalby, P. A., Ward, J. M. & Aeppli, G. Virus lasers for biological detection. Nat. Commun. 10, 3594 (2019).
Xu, Z. et al. Random lasing from label-free living cells for rapid cytometry of apoptosis. Nano Lett. 22, 172–178 (2022).
Fikouras, A. H. et al. Non-obstructive intracellular nanolasers. Nat. Commun. 9, 4817 (2018).
Lv, Z. et al. Intracellular near-infrared microlaser probes based on organic microsphere-SiO2 core-shell structures for cell tagging and tracking. ACS Appl. Mater. Interfaces 10, 32981–32987 (2018).
Tang, S.-J. et al. Laser particles with omnidirectional emission for cell tracking. Light Sci. Appl. 10, 23 (2021).
Tang, S. K. Y. et al. A multi-color fast-switching microfluidic droplet dye laser. Lab Chip 9, 2767–2771 (2009).
Richter, D., Marinčič, M. & Humar, M. Optical-resonance-assisted generation of super monodisperse microdroplets and microbeads with nanometer precision. Lab Chip 20, 734–740 (2020).
Humar, M., Muševič, I., Sullivan, K. G. & Hall, D. G. 3D microlasers from self-assembled cholesteric liquid-crystal microdroplets. Opt. Express 18, 26995–27003 (2010).
Chen, Y. C. et al. Monitoring neuron activities and interactions with laser emissions. ACS Photonics 7, 2182–2189 (2020).
Wu, X. et al. Nanowire lasers as intracellular probes. Nanoscale 10, 9729–9735 (2018).
Hua, B., Motohisa, J., Kobayashi, Y., Hara, S. & Fukui, T. Single GaAs/GaAsP coaxial core−shell nanowire lasers. Nano Lett. 9, 112–116 (2009).
Feng, C. et al. Organic-nanowire-SiO2 core-shell microlasers with highly polarized and narrow emissions for biological imaging. ACS Appl. Mater. Interfaces 9, 7385–7391 (2017).
Fernandez-Bravo, A. et al. Continuous-wave upconverting nanoparticle microlasers. Nat. Nanotechnol. 13, 572–577 (2018).
Wang, S. et al. High-yield plasmonic nanolasers with superior stability for sensing in aqueous solution. ACS Photonics 4, 1355–1360 (2017).
Caixeiro, S., Gaio, M., Marelli, B., Omenetto, F. G. & Sapienza, R. Silk-based biocompatible random lasing. Adv. Opt. Mater. 4, 998–1003 (2016).
Mysliwiec, J., Cyprych, K., Sznitko, L. & Miniewicz, A. Biomaterials in light amplification. J. Opt. 19, 033003 (2017).
Cho, S., Yang, Y., Soljačić, M. & Yun, S. H. Submicrometer perovskite plasmonic lasers at room temperature. Sci. Adv. 7, 3362–3387 (2021).
Liu, P. Y. et al. Cell refractive index for cell biology and disease diagnosis: past, present and future. Lab Chip 16, 634–644 (2016).
Chan, K. K. et al. Monitoring amyloidogenesis with a 3D deep-learning-guided biolaser imaging array. Nano Lett. 22, 8949–8956 (2022).
Chen, Q. et al. Highly sensitive fluorescent protein FRET detection using optofluidic lasers. Lab Chip 13, 2679–2681 (2013).
Oki, O. et al. FRET-mediated near infrared whispering gallery modes: studies on the relevance of intracavity energy transfer with Q-factors. Mater. Chem. Front. 2, 270–274 (2018).
Yuan, Z., Wang, Z., Guan, P., Wu, X. & Chen, Y. C. Lasing-encoded microsensor driven by interfacial cavity resonance energy transfer. Adv. Opt. Mater. 8, 1–9 (2020).
Wang, Y. et al. Demonstration of intracellular real-time molecular quantification via FRET-enhanced optical microcavity. Nat. Commun. 13, 6685 (2022).
Li, X. et al. Optical coherence tomography and fluorescence microscopy dual-modality imaging for in vivo single-cell tracking with nanowire lasers. Biomed. Opt. Express 11, 3659–3672 (2020).
Gratton, S. E. A. et al. The effect of particle design on cellular internalization pathways. Proc. Natl Acad. Sci. USA 105, 11613–11618 (2008).
Stewart, M. P. et al. In vitro and ex vivo strategies for intracellular delivery. Nature 538, 183–192 (2016).
Stewart, M. P., Langer, R. & Jensen, K. F. Intracellular delivery by membrane disruption: mechanisms, strategies, and concepts. Chem. Rev. 118, 7409–7531 (2018).
Wu, Y. C. et al. Massively parallel delivery of large cargo into mammalian cells with light pulses. Nat. Methods 12, 439–444 (2015).
Cho, S., Humar, M., Martino, N. & Yun, S. H. Laser particle stimulated emission microscopy. Phys. Rev. Lett. 117, 193902 (2016).
Humar, M., Upadhya, A. & Yun, S. H. Spectral reading of optical resonance-encoded cells in microfluidics. Lab Chip 17, 2777–2784 (2017).
Dannenberg, P. H. et al. Laser particle activated cell sorting in microfluidics. Lab Chip 22, 2343–2351 (2022).
Bednarkiewicz, A., Chan, E. M., Kotulska, A., Marciniak, L. & Prorok, K. Photon avalanche in lanthanide doped nanoparticles for biomedical applications: super-resolution imaging. Nanoscale Horiz. 4, 881–889 (2019).
Hill, M. T. & Gather, M. C. Advances in small lasers. Nat. Photonics 8, 908–918 (2014).
Dannenberg, P. H. et al. Droplet microfluidic generation of a million optical microparticle barcodes. Opt. Express 29, 38109–38118 (2021).
Liapis, A. C. et al. Conformal coating of freestanding particles by vapor-phase infiltration. Adv. Mater. Interfaces 7, 2001323 (2020).
Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676–682 (2012).
Ferrand, P. GPScan.VI: a general-purpose LabVIEW program for scanning imaging or any application requiring synchronous analog voltage generation and data acquisition. Comput. Phys. Commun. 192, 342–347 (2015).
Sofroniew, N. et al. napari: a multi-dimensional image viewer for python. Zenodo http://doi.org/10.5281/zenodo.8115575 (2022).
Allan, D. B., Caswell, T., Keim, N. C., van der Wel, C. M. & Verweij, R. W. soft-matter/trackpy: Trackpy v0.6.1. Zenodo http://doi.org/10.5281/zenodo.7670439 (2021).
Titze, V. M., Caixeiro, S., Schubert, M. & Gather, M. C. GatherLab/sphyncs. Zenodo http://doi.org/10.5281/zenodo.8121099 (2023).
Titze, V. M. et al. Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers (dataset). Available at http://doi.org/10.17630/bec9180a-86e6-4157-822f-fa84249452dd (2023).
Caixeiro, S. et al. Micro and nano lasers from III-V semiconductors for intracellular sensing. In Enhanced Spectroscopies and Nanoimaging 2020 Vol. 11468, 1146811 (eds. Verma, P. & Suh, Y. D.) (SPIE, 2020).
Rübsam, M. et al. E-cadherin integrates mechanotransduction and EGFR signaling to control junctional tissue polarization and tight junction positioning. Nat. Commun. 8, 1250 (2017).
Dannenberg, P. H. et al. Facile layer-by-layer fabrication of semiconductor microdisk laser particles. APL Photonics 8, 021301 (2023).
Yu, D. et al. Whispering-gallery-mode sensors for biological and physical sensing. Nat. Rev. Methods Prim. 1, 83 (2021).

Hyperspectral confocal imaging for high-throughput readout and analysis of bio-integrated microlasers

Supplementary information

Supplementary Information

Reporting Summary

Supplementary Video

推荐阅读