Imaging and optogenetic modulation of vascular mural cells in the live brain

Lei Tong, Robert A. Hill, Eyiyemisi C. Damisah, Katie N. Murray, Peng Yuan, Angelique Bordey, Jaime Grutzendler

Published: 2020-12-08 DOI: 10.1038/s41596-020-00425-w

vascular mural cells

optogenetic modulation

intravital imaging

blood–brain barrier

neurodegenerative diseases

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Mural cells (smooth muscle cells and pericytes) are integral components of brain blood vessels that play important roles in vascular formation, blood–nobreakbrain barrier maintenance, and regulation of regional cerebral blood flow (rCBF). These ce
Introduction
Materials
Procedure 1: Mural cell labeling and imaging
Procedure 2: Mural cell physiology
Procedure 3: Mural cell optogenetics
Troubleshooting
Timing
Anticipated results
References
ACKNOWLEDGMENTS

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Imaging and optogenetic modulation of vascular mural cells in the live brain

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