Establishment of human fetal hepatocyte organoids and CRISPR–Cas9-based gene knockin and knockout in organoid cultures from human liver

Delilah Hendriks, Benedetta Artegiani, Huili Hu, Susana Chuva de Sousa Lopes, Hans Clevers

Published: 2020-11-26 DOI: 10.1038/s41596-020-00411-2

CRISPR-Cas9

fetal hepatocyte organoids

gene knockin

gene knockout

organoid cultures

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The liver is composed of two epithelial cell types: hepatocytes and liver ductal cells. Culture conditions for expansion of human liver ductal cells invitro as organoids were previously described in a protocol; however, primary human hepatocytes remained
Introduction
Materials
Procedure 1 (Human fetal hepatocyte organoid line establishment and genome engineering)
Procedure 2 (Genome engineering of human liver ductal organoids)
Procedure 3 (Genotyping, further genome engineering and downstream applications)
Troubleshooting
Timing
Anticipated results
- Reporting Summary
References
ACKNOWLEDGMENTS

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Establishment of human fetal hepatocyte organoids and CRISPR–Cas9-based gene knockin and knockout in organoid cultures from human liver

Supplementary information

Supplementary Information

Reporting Summary

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