Top agar: 0.7% w/v LB(Miller) agar
HANNAH ZHU
Published: 2023-11-29 DOI: 10.17504/protocols.io.eq2lyj2oelx9/v1
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Abstract
how to make Top agar for plaque assay experiment
Steps
Top agar: 0.7% w/v LB(Miller) agar
1.
Dissolve following components in 200 mL MilliQ water
| A | B | C |
|---|---|---|
| Tryptone | 1% w/v | 2 g |
| Yeast extract | 0.5% w/v | 1 g |
| NaCl | 1% w/v | 2 g |
| Agar | 0.7% | 1.4 g |
2.
Autoclave at 121°C for 0h 15m 0s
3.
Store in oven (> 50°C)
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