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    Volume of oils (fatty acids) in coffee and comparison between roast levels

    Shannon.logan

    Published: 2023-04-27 DOI: 10.17504/protocols.io.14egn2kozg5d/v1
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    Abstract

    Measuring the volume of oils produced in coffee across a range of roasts.

    Steps

    1.

    Grind coffee to desired consistency and weigh 20g to put into cafetiere

    2.

    Boil kettle with 250ml of deionised water

    3.

    Pour boiling water into a beaker and allow to cool to 93$$°C, using a digital thermometer to ensure accuracy of temperature.

    4.

    Once the water has cooled to the desired temperature, pour into the cafetiere and allow to brew for 4 minutes, using a timer/stopclock.

    5.

    Pour each 2 of each sample into the centrifuge tubes, and centrifuge samples at 6000rpm for 30 minutes.

    6.

    When the centrifugation is complete, using a syringe, remove the top layer of oils from the sample, and measure against the syringe measurements.

    7.

    Take note of each sample's volume.

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